IL8 ELISA Kit for Chicken(Gallus gallus) | EIAab.comIL8 ELISA Kit for Chicken(Gallus gallus)

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Index > Protein center > IL8(Gene name) > Chicken

IL8 (Gene name),

Interleukin-8 (Protein name ), IL8_CHICK from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

IL8(CEF4;CXCL8;EMF1);

Protein name:

Interleukin-8(IL-8);

Alternative:

C-X-C motif chemokine 8;9E3;Embryo fibroblast protein 1(EMF-1);CEF-4;

Organism:

Chicken (Gallus gallus).

General Annotation

Sub Unit:

N/A

Function:

May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

103

Sequence:

50:

100:

103:

APL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P08317

UniGene:

Gga.826

KEGG:

gga:396495

Pfam:

PF00048

Uniprot:

P08317

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ELISA Kit for Chicken IL-8

Cat.:

E0080c

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96T

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ELISA Kit for Chicken IL-8

Cat.:

E0080p

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96T

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ELISA Kit for Chicken IL-8

Cat.:

E0080h

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ELISA Kit for Chicken IL-8

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ELISA Kit for Chicken IL-8

Cat.:

E0080d

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ELISA Kit for Chicken IL-8

Cat.:

E0080b

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96T

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CLIA Kit for Chicken IL-8

Cat.:

U0080b

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96T

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CLIA Kit for Chicken IL-8

Cat.:

U0080d

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96T

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CLIA Kit for Chicken IL-8

Cat.:

U0080c

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96T

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CLIA Kit for Chicken IL-8

Cat.:

U0080h

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Looking for manual.

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96T

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CLIA Kit for Chicken IL-8

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U0080p

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CLIA Kit for Chicken IL-8

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Polyclonal Antibody for Chicken IL-8

Cat.:

P0080Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Chicken IL-8

Protein for Chicken IL-8

Cat.:

R0080h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Chicken IL-8

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.6%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-106
Plasma	101	95-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-106
Plasma	104	102-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	97-104%	95-101%	103-106%
EDTA plasma(n=5)	98-104%	94-103%	90-97%	105-109%
heparin plasma(n=5)	94-99%	103-108%	96-104%	98-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-103%	92-105%	95-106%	94-109%
EDTA plasma(n=5)	89-95%	93-103%	94-102%	95-106%
heparin plasma(n=5)	93-105%	94-107%	101-106%	96-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-102%	103-107%	97-105%	96-103%
EDTA plasma(n=5)	100-104%	99-107%	102-108%	97-102%
heparin plasma(n=5)	104-106%	94-102%	108-112%	97-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Transformation by Rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein."
Sugano S. , Stoeckle M.Y. , Hanafusa H.
Cell49:321-328(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Constitutive expression of a gene encoding a polypeptide homologous to biologically active human platelet protein in Rous sarcoma virus-transformed fibroblasts."
Bedard P.-A. , Alcorta D. , Simmons D. , Luk K.-C. , Erikson R.L.
Proc. Natl. Acad. Sci. U.S.A.84:6715-6719(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"The chicken 9E3/CEF4 CXC chemokine is the avian orthologue of IL8 and maps to chicken chromosome 4 syntenic with genes flanking the mammalian chemokine cluster."
Kaiser P. , Hughes S. , Bumstead N.
Immunogenetics49:673-684(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

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